MOLECULAR IDENTIFICATION AND PHYLOGENETIC ANALYSIS OF MASTOPHORUS MURIS ISOLATES FROM MICROTUS FUSCUS
10.3969/j.issn.1005-0507.2023.04.002
- VernacularTitle:青海田鼠源鼠突线虫的分子鉴定与进化分析
- Author:
Zheng-He SHI
1
;
Yong FU
;
Xue-Yong ZHANG
;
Jie CAO
;
Ru MENG
;
Wen-Hai BIE
;
Yu-Jiao GU
;
Yan-Ying JIN
;
Hong-Bin WANG
Author Information
1. 青海大学临床医学院, 西宁 810016
- Keywords:
Microtus fuscus;
Mastophorus muris;
Molecular identification;
Germline development
- From:
Acta Parasitologica et Medica Entomologica Sinica
2023;30(4):197-201
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to identify the species of nematodes originating from Microtus fuscus.Mitochondrial COI gene sequences of the nematode samples were amplified using PCR,and the resulting amplification products were sequenced.The obtained sequences were compared and subjected to phylogenetic analysis,revealing that the nematode species isolated from M.fuscus is identical to Mastophorus muris,exhibiting a high homology of 98.6% with M.muris(MK8674).The germline evolutionary tree demonstrated that the isolate is distantly related to other nematodes documented in GenBank,but shares a branch with M.muris(MK8674),representing its closest relative.This nematode belongs to the rat-parasitic nematodes of the stomach.This study marks the first molecular level identification of M.muris from Qinghai voles,providing a scientific basis for further epidemiological investigations of M.muris on the Qinghai-Tibet Plateau.
