STUDY ON LF-RPA DETECTION METHOD OF PLASMODIUM FALCIPARUM
10.3969/j.issn.1005-0507.2023.04.001
- VernacularTitle:恶性疟原虫LF-RPA检测方法研究
- Author:
Jing-Yu GAO
1
;
Qiu-Yang LI
;
Yao YAO
;
Yuan-Hong XIE
;
Yan-Hua LIU
Author Information
1. 中国海关科学技术研究中心, 北京 100026
- Keywords:
Plasmodium falciparum;
Recombinase polymerase amplification;
Lateral flow;
Infectious disease;
Rapid detection
- From:
Acta Parasitologica et Medica Entomologica Sinica
2023;30(4):193-196,209
- CountryChina
- Language:Chinese
-
Abstract:
To establish a novel detection technique for Plasmodium falciparum on the combination of recombinase polymerase amplification(RPA)and lateral flow(LF)chromatography,the primer sets and probes were designed according to the conserved region of 18S rDNA in P.falciparum,and the detection protocol were organized following the assessment of sensitivity and specificity.As results,the newly developed protocol detect P.falciparum DNA effectively within 15 min at 39℃,which indicates the protocol with good specificity and sensitivity,the lower detection limit is near to 6.54×102 copies/reaction,much sensitive than commercial colloidal gold test strip method.The protocol established should be applicable in the rapid and accuracy detection of P.falciparum in clinical and related samples.
