AN ALTERNATIVE CELLULAR MODEL FOR GENETIC VARIATIONS OF JAPANESE ENCEPHALITIS VIRUS IN THE TRANSMISSION PROCESS BETWEEN VERTEBRATES AND MOSQUITOES
10.3969/j.issn.1005-0507.2022.02.003
- VernacularTitle:哺乳动物细胞与蚊虫细胞交替传代培养模型用于乙脑病毒基因变异规律的研究
- Author:
Wen-Jun HOU
1
;
Yu-Ting JIANG
;
Heng-Duan ZHANG
;
Chun-Xiao LI
;
Dan XING
;
Xiao-Xia GUO
;
Tong-Yan ZHAO
Author Information
1. 军事科学院军事医学研究院微生物流行病研究所病原微生物生物安全国家重点实验室,北京市虫媒病与自然疫源性疾病重点实验室,北京100071;武警部队特色医学中心研究部突发公共卫生事件医学防治研究所,北京100612
- Keywords:
JEV;
Alternative passage;
Genetic variation;
Fitness evaluation;
Cell model
- From:
Acta Parasitologica et Medica Entomologica Sinica
2022;29(2):77-84,115
- CountryChina
- Language:Chinese
-
Abstract:
To simulate alternative microenvirmonental habitats in vertebrates and mosquito hosts,an in vitro model was utilized to determine the gene variations'pattern of the Japanese encephalitis virus(JEV).Different JEV-SA14 mutants were obtained and compared after sequenced,and the results showed that variable loci had been found in JEV during passaging in different microenviroments.All mutations were found single-based without any insertions or deletions,which suggests that the highly conserved evolution of JEV under the natural selections during viral transmission.The fitness of JEV to different hosts evaluated were shown comparable to previous studies along with corresponding genetic mutations.The in vitro alternative passaging protocol appeared to be reliable to simulate the variant infection microenviromental habitats between vertebrated hosts and vectorial mosquitoes.
