RAPID DETECTION METHOD OF HANTAVIRUS SEOV-S4 BASED ON ENZYMATIC PROBE ISOTHERMAL AMPLIFICATION

VernacularTitle:基于酶切探针等温扩增技术快速检测汉坦病毒S4亚型方法的建立
Author: Jia-Qiao LI ¹ ; Le-Le AI ; Chang-Qiang ZHU ; Ling-Ling ZHANG ; Hua-Gui WANG ; Yi-Zhe LUO ; Qi TANG ; Yang ZHOU ; Wei-Long TAN
Author Information

1. 东部战区疾病预防控制中心,江苏南京 210002;福建农林大学,福建福州 350002
Keywords: Enzymatic Probe Isothermal Amplification(EPIA); Hantavirus; Detection method
From: Acta Parasitologica et Medica Entomologica Sinica 2021;28(4):243-249
CountryChina
Language:Chinese
Abstract: Hantavirus(HV)belongs to Seoul virus-S4 subtype(SEOV-4),which is the main epidemic subtype of HV in southeast coastal area.In this study,a rapid detection method for SEOV-S4 was established based on Enzymatic Probe Isothermal Amplification(EPIA)and primers and probes were designed to target the relatively conserved S sequence of SEOV-S4.The sensitivity and repeatability were verified by gradient dilution of target gene synthetic plasmid,and the specificity was verified by other human subtype HV.The results showed that the sensitivity of SEOV-S4 method based on EPIA could reach 10 copies/μL.There was no cross-reaction with other human subtypes of HV.The method established in this study has good sensitivity,specificity and repeatability,and can be used for the diagnosis and screening of relevant samples.