CARBOXYLESTERASE GENE OVEREXPRESSION AND DELTAMETHRIN RESISTANCE IN CULEX PIPIENS PALLENS
10.3969/j.issn.1005-0507.2021.04.005
- VernacularTitle:淡色库蚊羧酸酯酶基因高表达与溴氰菊酯抗药性相关性研究
- Author:
Yuan LYU
1
;
Fan QIU
;
Hong-Bo ZHANG
;
Jing-Wei XU
;
Bo SHEN
Author Information
1. 南京市第一医院,南京医科大学附属南京医院核医学科,江苏 南京 210006
- Keywords:
Culex pipiens pallens;
Deltamethrin;
Carboxylesterase;
Fluorescence quantitative PCR;
Field resistance detection
- From:
Acta Parasitologica et Medica Entomologica Sinica
2021;28(4):231-236
- CountryChina
- Language:Chinese
-
Abstract:
Fluorescence quantitative PCR method was used to detect the relative expression levels of carboxylesterase gene(CPIJ002786-RA)in sensitive and resistant strains of Culex pipiens pallens of both laboratory population(LC50=0.03 mg/L and 2.00 mg/L by larval bioassay),and sensitive and resistant strains(differentiated by WHO susceptibility tube bioassay)in 4 field populations(Shanghe/Gudao/Huimin of Shandong Province,Jiangxinzhou of Jiangsu Province).The expression level of carboxylesterase(CPIJ002786-RA)was 2.77-fold higher in resistant strains than in susceptible strains of laboratory populations(P＜0.01),and were 3.65-,5.67-and 2.75-fold higher in three resistant strains of field populations(Shanghe,Gudao and Huimin)than in susceptible strains(P＜0.05).However,the expression level of carboxylesterase was no significant difference between resistant and sensitive strains(P＞0.05)in Jiangxinzhou field population.The over expression of carboxylesterase gene(CPIJ002786-RA)in Cx.pipiens pallens may be closely related to the deltamethrin resistance,which means a potential application value as an index gene in field resistance detection.
