MOLECULAR CHARACTERIZATION OF TWO VARIANT TOTIVIRUSES ISOLATED FROM CULEX TRITAENIORHYNCHUS IN CHINA
10.3969/j.issn.1005-0507.2020.01.004
- VernacularTitle:两株从三带喙库蚊中分离的toti病毒变异株的分子特征
- Author:
Hong-Hong PENG
1
;
Yuan-Yuan WANG
;
Ke DAI
;
Qiu-Min ZHAO
;
Shu-Qing ZUO
;
Yi-Gang TONG
;
Jiu-Song ZHANG
Author Information
1. 安徽医科大学
- Keywords:
Totivirus;
Virus isolation;
High-throughput sequencing;
Complete genome
- From:
Acta Parasitologica et Medica Entomologica Sinica
2020;27(1):20-25
- CountryChina
- Language:Chinese
-
Abstract:
Two totiviruses, temporarily named Totivirus ND3 (TOV_ND3) and FD17 (TOV_FD17), were isolated from Culex tritaeniorhynchus captured in Shanghai City. The full sequences were determined using high-throughput sequencing method. The results showed that the genomes of TOV_ND3 and FD17 were 7612 and 7611 nucleotides, respectively in length, and encoded a capsid protein and an RNA-dependent RNA polymerase. The nucleotide sequence of TOV_ND3 had the highest similarity to Cx. tritaeniorhynchus totivirus NJ2, while the FD17 was most similar to Omono River virus strain AK4, with 84. 6％ and 84. 9％ identity, respectively, and 74. 1％ identity between themselves. Phylogenetic analysis revealed that the two newly obtained viruses were grouped into the unassigned totiviruses. Our results suggested that the two new viruses were variant totiviruses.
