SIMULTANEOUS AND RAPID DETECTION OF FOUR KINDS OF PATHOGENIC RICKETTSIA BY QUANTITATIVE REAL-TIME PCR
10.3969/j.issn.1005-0507.2019.02.007
- VernacularTitle:实时荧光定量PCR同时快速检测4类致病性立克次体?
- Author:
Yong-Hui YU
1
;
Jun JIAO
;
Meng-Jiao FU
;
Lu-Peng DAI
;
Jun-Xia FENG
;
Xue-Yuan HU
;
Bo-Hai WEN
;
Dong-Sheng ZHOU
;
Xiao-Lu? XIONG
Author Information
1. 军事科学院军事医学研究院微生物流行病研究所
- Keywords:
Rickettsias;
Quantitative real-time PCR;
Simultaneous detection
- From:
Acta Parasitologica et Medica Entomologica Sinica
2019;26(2):109-116
- CountryChina
- Language:Chinese
-
Abstract:
To establish a rapid detection method for four kinds of common pathogenic Rickettsia, the primers and probes were designed according to the sequences of lgtD gene of typhus group rickettsia, ompB gene of speckle-heat group rickettsia, Orientalis tsutsugamushi gene and 23S rRNA gene of Coxiella burnetii. Using the cloned gene fragment as DNA template, a four-module real-time fluorescence quantitative PCR method was established. The specificity and sensitivity of the method were evaluated by nucleic acid samples of different rickettsia and bacterial strains. The cyclic threshold (Ct) of the standard curves of the four modules showed a good linear relationship with the number of copies of the template, and had good sensitivity, specificity and reproducibility in detecting the corresponding samples of Rickettsia nucleic acid. The newly established method was suitable for the rapid detection of trace rickettsia DNA, and for clinical laboratory use in rapid diagnosis of Rickettsia disease.
