DNA BARCODING AND PHYLOGENETIC RELATIONSHIP OF PARTIAL SPECIES OF POLLENIA INTERCEPTED AT SHANGHAI PORT
10.3969/j.issn.1005-0507.2019.01.007
- VernacularTitle:上海口岸截获的粉蝇属DNA条形码鉴定及系统发育研究?
- Author:
Juan DU
1
;
Yao-Hua DENG
;
Zi-Long ZHANG
;
Tong-Yan ZHAO
;
Shen-Wei LI
;
Zhen-Gan TIAN
Author Information
1. 上海国际旅行卫生保健中心
- Keywords:
COⅠ gene;
DNA barcoding;
Pollenia;
Frontier port
- From:
Acta Parasitologica et Medica Entomologica Sinica
2019;26(1):41-46
- CountryChina
- Language:Chinese
-
Abstract:
Species identification with dry specimen is usually difficult by traditional taxonomy method. According to the operational regulations for DNA barcode identification of medical insects at frontier ports, DNA barcodes of 31 dry specimens of Pollenia species captured at Shanghai port were generated. After comparing with the Barcode of Life Data (BOLD) system v4, all the 31 DNA barcodes are assigned into 5 species: Pollenia rudis (Fabricius, 1794), Pollenia pediculata (Macquart, 1834), Pollenia vagabunda (Meigen, 1826), Pollenia hungarica ( Rognes, 1987) and Pollenia sp.. A neighbor-joining tree was created based on the 31 barcodes of Pollenia and an inductive COⅠ sequence as an outgroup, it is revealed that these species can be differentiated by DNA barcodes. Therefore, COⅠ barcoding can overcome the shortages of traditional taxonomy, and improve the success rate in identification of flies with dry specimen.
