RAPID DETECTION OF CHIKUNGUNYA FEVER VIRUS WITH REVERSE TRANSCRIPTION RECOMBINASE AIDED AMPLIFICATION
10.3969/j.issn.1005-0507.2017.04.002
- VernacularTitle:逆转录重组酶介导扩增技术快速检测基孔肯雅热病毒
- Author:
Peng LUO
1
;
Zhi-Fan JIANG
;
Wei ZHENG
;
Zhong-Hua WU
;
Qin-Feng LYU
;
Li-Chuan GUO
;
Qing-Jie YING
;
Gang WANG
Author Information
1. 浙江国际旅行卫生保健中心
- Keywords:
Recombinase aided amplification;
Chikungunya virus;
Molecular detection
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(4):212-216
- CountryChina
- Language:Chinese
-
Abstract:
The Chikungunya fever virus (CHIKV) RNA was first transcribed into the cDNA by a reverse-transcriptase reaction,and the resulting product was then served as template for RT-RAA amplification. Universal primers and probe were designed according to the conserved genome sequence of the CHIKV to evaluate the specificity and sensitivity of the methods and develop a rapid one-step reverse transcription recombinase aided amplification (RT-RAA) assay for (CHIKV) detection. The reaction was performed at a constant temperature of 39℃ with a short detection time ( <20 minutes). Our results indicated that the amplification is very sensitive which can detect 100 copies. The approach is also shown very specific as it had no cross reaction with other related viruses,such as Dengue virus,West Nile virus,Japanese encephalitis virus or Yellow fever virus. This RT-RAA assay was rapid,specific and sensitive,and might be utilized in rapid surveillance for CHIKV on ports.
