DETECTION AND SEQUENCES ANALYSIS OF CANDIDATUS RICKETTSIA BARBARIAE IN RHIPICEPHALUS TICKS FROM XINJIANG
10.3969/j.issn.1005-0507.2017.03.001
- VernacularTitle:新疆地区扇头蜱中Candidatus Rickettsia barbariae的检测与序列分析
- Author:
Xiang SUN
1
;
Gui-Lin ZHANG
;
Zhong ZHENG
;
Ran LIU
;
Er-Chen QIU
Author Information
1. 新疆军区疾病预防控制中心
- Keywords:
Rhipicephalus sanguineus;
Rhipicephalus turanicus;
Candidatus Rickettsia barbariae;
Spotted fever group rickettsia;
Xinjiang
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(3):141-147
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the prevalence of Candidatus Rickettsia barbariae in ticks from Xinjiang,total of 90 Rhipicephalus sanguineus and 420 Rhipicephalus turanicus were collected from Yuli area and the genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detect with PCR.The target genes of ompA,ompB,gltA and 17kDa of Candidatus Rickettsia barbariae were detected from 10 Rh.sanguineus and 53 Rh.turanicus with rates as 11.11％ and 12.62％ respectively.The sequences obtained from these ticks were identical to the corresponding sequence in the positive samples.By BLAST analysis,the ompA,ompB partial sequences were 99.8％ (583/584) and 100％ (768/768) identical to the corresponding sequence of Candidatus Rickettsia barbariae strain from Rh.turanicus in Italy (EU272186.1,EU272187.1) respectively.The gltA and 17kDa partial sequence were also 100％ identical (375/375,356/356) to the corresponding sequence of Candidatus Rickettsia barbariae strain from Vermipsylla alakurt in Xinjiang (KT284716.1,KT284715.1).The manifestations of Candidatus Rickettsia barbariae occur in Rh.sanguineus and Rh.turanicus might suggest the potential health threat of the rickettsia organism to residents in Xinjiang area.
