ESTABLISHMENT AND APPLICATION OF VISIBLE RT-LAMP DETECTION METHOD FOR ZIKA VIRUS
10.3969/j.issn.1005-0507.2017.02.006
- VernacularTitle:寨卡病毒可视化RT-LAMP检测方法的建立与应用
- Author:
Le-Le AI
1
;
Chen-Xi DING
;
Xiu-Fang GONG
;
Chang-Qiang ZHU
;
Qi ZHANG
;
Yi-Fang HAN
;
Wei-Long TAN
;
Chang-Jun WANG
Author Information
1. 南京医科大学公共卫生学院
- Keywords:
Zika virus;
Reverse transcription loop mediated isothermal amplification;
Visual detection;
Hydroxy naphthol blue
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(2):103-108
- CountryChina
- Language:Chinese
-
Abstract:
The study established a simple,rapid and accurate reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for zika virus (ZIKV).Three sets of LAMP primers were designed with online tools.The visual RT-LAMP assay was established with the optimized primers selected by real-time turbidimeter and Hydroxy naphthol blue (HNB),which possessed high sensitivity and specificity.The detection limit of this method was 10 copies/reaction,which was 10-100 fold higher than the conventional PCR and quantitative real-time PCR.The method was high specificity without any cross-reaction with other arbovirus.This method provided an experimental tool for the arbovirus monitoring and clinical diagnosis.
