ESTABLISHMENT OF NEOSPOROSIS NUCLEIC ACID DETECTION METHODS AND PRIMARY APPLICATION
10.3969/j.issn.1005-0507.2017.02.003
- VernacularTitle:新孢子虫核酸检测方法的建立及应用评价
- Author:
Wen-Chen MA
1
;
Yun-Feng LI
;
Qi WANG
;
Ping LI
;
Kui ZHENG
;
Wei-Li WANG
;
Jing-Lin WANG
;
Da-Li ZHAO
;
Hong-Jiu ZOU
;
Ming-Hua JIN
Author Information
1. 吉林大学公共卫生学院
- Keywords:
Neospora caninum;
Genes;
Mice;
PCR
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(2):79-84
- CountryChina
- Language:Chinese
-
Abstract:
Establish a highly specific and sensitive PCR method to detect Neospora caninum.A set of primers was designed by targeting the Nc-5 gene of Neospora caninum.The specificity was assayed by testing different species of parasites (i.g.Toxoplasma gondii,Plasmodium,Theileria annulata,Bonamia,Marteilia refringens).The sensitivity of the method was test by series of to dilute DNA of Neospora caninum.The detection effect of this method was evaluated by using the mouse infection model established in the laboratory.The assay indicates PCR method is a potential and valuable means for Neospora caninum detection.The detection sensitivity of the PCR method is about 0.5788 pg/μL DNA.Moreover this method has high specificity,repeatable and stability.
