IMPEDIMETRIC IMMUNOSENSOR BASED ON NANOBODY AND GOLD NANOPARTICLES MODIFIED GRAPHENE PAPER FOR DETECTION OF SCHISTOSOMA JAPONICUM CIRCULATING ANTIGEN
10.3969/j.issn.1005-0507.2017.02.002
- VernacularTitle:基于纳米抗体和石墨烯/金复合材料的免疫传感器检测日本血吸虫循环抗原的研究
- Author:
Yi-Xiu FU
1
;
Qing-Ming KONG
;
Bin ZHENG
;
Di LOU
;
Jian-Zu DING
;
Pei ZHAO
;
Miao WANG
;
Shao-Hong LU
Author Information
1. 浙江省医学科学院
- Keywords:
Nanobody;
Graphene;
Schistosoma japonicum circulating antigen;
Immunosensor
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(2):70-78
- CountryChina
- Language:Chinese
-
Abstract:
In this study,a new type of impedimetric immunosensor was constructed based on graphene paper for detection of Schistosoma japonicum circulating antigen.The prepared graphene paper was modified with nanobody and gold nanoparticls and the surface morphology and crystal structure of the graphene paper was characterized by scanning electron microscope,atomic force microscope,raman spectroscopy.At the same time,specificity and sensibility of this immuosensor were analyzed.It is demonstrated that the developed impedimetric immunosensor possesses excellent specificity and sensibility,the detection level of S.japonicum soluble egg antigen(SEA) reaches 0.01 ng/mL;The detection rate of suspected schistosomiasis patients was 66.7%,which was better than that (56.7%) of ELISA,and the coincidence rate with ELISA was 90%.The immunosensor has high sensitivity and specificity for detecting S.japonicum circulating antigen.The method is simple,fast and has good potential for application.
