IDENTIFICATION AND EXPRESSION ANALYSIS OF ADENOSINE DEAMINASE GENE FAMILY OF GENES IN AEDES AEGYPTI
10.3969/j.issn.1005-0507.2017.01.003
- VernacularTitle:埃及伊蚊腺苷脱氨酶相关基因的鉴定和表达分析
- Author:
Qing-Qiao LYU
1
;
Hui ZHAI
;
Feng YAN
;
Jin-Zhi CHENG
;
Jia-Hong WU
;
Zheng-Ling SHANG
Author Information
1. 贵州医科大学环境污染与疾病监控省部共建教育部重点实验室
- Keywords:
Aedes aegypti;
ADA gene family;
qRT-PCR;
Expression profile
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(1):12-18
- CountryChina
- Language:Chinese
-
Abstract:
To identify the adenosine deaminase (ADA)-related genes in Ae.aegypti and their expression pattern at various developmental stages and in different tissues of female.The ADA-related genes were identified by an extensive search on the Ae.aegypti genome with the ADA domain of Drosophila melanogaster (GenBank No.NM_ 141609,NM_079406,AF384215,AF337552,AF337553) as clues.The domains were analyzed with the on-line tools,the signal peptides was predicted with Signal P4.1 software.Sequence alignment and the phylogenetic tree were made by MEGA 6.0 and GeneDoc.Specific primers for each ADA member were disigned and quantitative analysis was conducted by real-time RT-PCR.Three ADA-related genes with complete ORF were finally identified from Ae.aegypti genome (Aa-ADAL,Aa-ADGF-A,Aa-ADGF-B),and high similarity was found only in the eight conserved ADA enzyme active sites.The three ADA-related genes were all expressed at different developmental stages and in various tissues of female.Among them,Aa-ADAL was expressed higher in the second instar (7.88) and the ovary (8.05),Aa-ADGF-A was expressed higher in the third instar (7.25),while the highest relative expression amount of Aa-ADGF-B transcripts was observed in salivary gland of female (10.07).AaADGF-A and Aa-ADGF-B belonged to ADA2/ADGF subfamily,and Aa-ADAL was classified into ADAL subfamily.
