STIMULATION EFFECTS OF SOLUABLE EGGS AND SCHISTOSOMULUM ANTIGENS FROM SCHISTOSOMA JAPONICUM ON RAW264.7 CELLS
10.3969/j.issn.1005-0507.2017.01.001
- VernacularTitle:日本血吸虫可溶性虫卵和童虫抗原刺激RAW264.7巨噬细胞的初步分析
- Author:
Yuan-Xi SHEN
1
;
Zu-Hang ZHANG
;
Hong-Xiao HAN
;
Zhi-Qiang FU
;
Yang HONG
;
Jiao-Jiao LIN
Author Information
1. 上海师范大学生命与环境科学学院
- Keywords:
Schistosoma japonicum;
Macrophages;
Soluble egg antigen;
Schistosomulum antigen
- From:
Acta Parasitologica et Medica Entomologica Sinica
2017;24(1):1-6
- CountryChina
- Language:Chinese
-
Abstract:
In this study,we stimulated mouse macrophages RAW264.7 using different concentration of soluble schistosome egg antigen (SEA) and schistosomulum antigen (SSA) respectively,the observations showed that SEA could significantly stimulate the proliferation and differentiation of RAW264.7 cells (SI ＞ 2),and the morphology of the cells were changed dramatically and some of them were died.qPCR analysis revealed that the expression level of TNF-α、IL-6、IL-10 and CCL2 were significantly higher in SEA or SSA stimulated cell groups than that of the control group.The stimulation effects of macrophages by schistosome antigens may play an important role in the regulation of host's innate immune response and granuloma formation in schistosomiasis.
