EFFECT OF MUSCA DOMESTICA ANTIBACTERIAL PEPTIDES ON THE PROLIFERATION, INVASION AND MIGRATION OF HEPG2 CELLS
10.3969/j.issn.1005-0507.2016.01.009
- VernacularTitle:家蝇幼虫抗菌肽对肝癌HepG2细胞增殖、侵袭及迁移的影响?
- Author:
JiaXin XING
1
;
JuanJuan LIU
;
ShouMin NIE
;
Shan GUO
;
RuiJun ZHAO
;
JingXia CHENG
Author Information
1. 山西医科大学基础医学院
- Keywords:
Crude extracting;
Musca domestica;
HepG2 cells;
Antibacterial peptides;
Proliferation;
Invasion;
Migration
- From:
Acta Parasitologica et Medica Entomologica Sinica
2016;23(1):51-56
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the antitumor function of the housefly antibacterial peptide in a more comprehensive way, this article studied the antitumor function of housefly antimicrobial peptides on the tumor cell proliferation, invasion and migration. To induce antibacterial peptides, we use the needle to prick housefly then go through a process of low temperature centrifugal, solid phase extraction and freeze?drying to obtain antibacterial peptides crude extract, and then adjust antibacterial peptides to different concentrations to inhibit CCK8, cell migration and invasion experiment. The experiment show that housefly antimicrobial peptide can inhibit HepG2 cells proliferation, and the dose?response characteristics is closely effected by its concentration. Low concentration of antibacterial peptides (60μg/mL) can also effectively inhibit the migration and invasion of HepG2 cells. In conclusion, the crude extractings of antibacterial peptides from housefly larvae can inhibit the proliferation, invasion and migration of HepG2 cells.
