A RECOMBINASE AIDED AMPLIFICATION ASSAY FOR PLASMODIUM
10.3969/j.issn.1005-0507.2016.01.003
- VernacularTitle:重组酶介导恒温扩增技术检测疟原虫方法的建立?
- Author:
Wei ZHENG
1
;
Gang WANG
;
YongYao YANG
;
Peng LUO
;
Juan FENG
;
LiChuan GUO
;
QingJie YING
Author Information
1. 浙江国际旅行卫生保健中心
- Keywords:
Recombinase aided amplification;
Plasmodium;
Molecular detection;
Malaria
- From:
Acta Parasitologica et Medica Entomologica Sinica
2016;23(1):15-20
- CountryChina
- Language:Chinese
-
Abstract:
To establish a recombinase aided amplification ( RAA) assay for Plasmodium, we use the RAA assay to screen Plasmodium. Universal primers and probe were designed based on the specific fragment of the 18S rDNA gene of Plasmodium. We screened the primers and detected the specificity of the method. We screened four better amplification primers. RAA was performed at 37℃ in a short time (40 minutes) with good specificity. The RAA assay for Plasmodium detection appears to be applicable in ports.
