ANALYSES OF SPECIFICITY AND SENSITIVITY OF ENZYME-LINKED IMMUNOSORBENT ASSAY WITH THREE MEMBRANE-ASSOCIATED PROTEINS OF ORIENTIA TSUTSUGAMUSHI
10.3969/j.issn.1005-0507.2015.03.001
- VernacularTitle:恙虫病东方体三种膜蛋白抗原包被酶联免疫吸附试验的特异性和敏感性分析
- Author:
Peng-Cheng WANG
1
;
Xiao-Lu XIONG
;
Jun JIAO
;
Wen-Ping GONG
;
Xiao-Mei YANG
;
Bo-Hai WEN
Author Information
1. 军事医学科学院微生物流行病研究所
- Keywords:
Orientia tsutsugamushi;
Tsutsugamushi disease;
Outer membrane protein;
Enzyme-linked immunosorbent assay
- From:
Acta Parasitologica et Medica Entomologica Sinica
2015;(3):141-146
- CountryChina
- Language:Chinese
-
Abstract:
The enzyme-linked immunosorbent assay ( ELISA) with a recombinant outer membrane protein ( r47, r56, or r58 kDa) of Orientia tsutsugamushi was analyzed for specificity and sensitivity in detection of specific Ig G antibodies in sera from mice and patients suffered from O.tsutsugamushi infection.As a result, all of 8 serum specimens from mice experimentally infected with O.tsutsugamushi positively reacted with r47, 56 and r58 kDa protein respectively, except for that one serum negatively reacted with r58 kDa, while all of 8 sera from mice infected with Coxiella burnetii, Rickettsia mooseri or R.heilongjiangensis negatively reacted with the three proteins.In addition, 11 of 12 sera from patients with tsutsugamushi disease positively reacted with the three proteins, respectively, while 11 sera from patients with Q fever, 11 sera from patients with endemic typhus, and 13 sera from patients with spotted fever positively reacted to r47 kDa in the ratios of 28%, 18%, and 23%, to r56 kDa in the ratios of 28%, 0%, and 8%, and to r58 kDa in the ratios of 18%, 18%, and 23%, respectively.The results suggest that the ELISA coated with r47, r56, or r58 kDa has a good specificity and sensitivity for serological diagnosis of tsutsugamushi disease.
