EFFECT OF THE CONCENTRATION OF MUSCA DOMESTICA ANTIMICROBIAL PEPTIDE ON THE PROLIFERATION OF PRIMARY FBL-3 CELLS
10.3969/j.issn.1005-0507.2015.01.005
- VernacularTitle:家蝇抗菌肽对 FBL－3红白血病原代细胞增殖影响浓度的探索
- Author:
Bin DU
1
;
Rui-Jun ZHAO
;
Jing-Xia CHENG
;
Wen-Jian WANG
;
Fa-Jia YUAN
;
Shou-Min NIE
;
Yan-Hong LI
Author Information
1. 山西医科大学基础医学部
- Keywords:
Antimicrobial peptide;
Housefly;
FBL-3 primary cell;
Concentration
- From:
Acta Parasitologica et Medica Entomologica Sinica
2015;(1):24-28
- CountryChina
- Language:Chinese
-
Abstract:
The inhibition effect of antimicrobial peptides from the housefly ( Musca domestica) larvae on primary cell FBL-3 was tested under laboratory.Antibacterial peptides extracts was purified with C18 solid phase methods from induced housefly larva.Erythroleukemia mice model was prepared by injected 2.25 ×106/mL FBL-3 cells into C57BL/6 mouse via the tail vein.The primary culture of FBL-3 cells was harvested from the mice and utilized to test the inhibition effect of the antibacterial peptide extracts.When observed with trypan blue staining methods, antibacterial peptide mixture at a concentration of 250-350 μg/mL affects FBL-3 primary cells significantly with increased cell debris and more mortality rate.Cell apoptosis was also shown in the concentration range tested with CCK-8 kit.The reference concentration of antibacterial peptide would be applied in further tests in vivo.
