CRUDE EXTRACTS AND PURIFIED PRODUCTS OF ANTIMICROBIAL PEPTIDES FROM HOUSEFLY LARVAEONSUP-B15 CELLPROLIFERATION INHIBITION AND INDUCE APOPTOSIS
10.3969/j.issn.1005-0507.2015.01.004
- VernacularTitle:家蝇幼虫抗菌肽粗提物与8种纯化物对SUP－B15细胞增殖抑制和诱导的凋亡作用
- Author:
Wen-Jian WANG
1
;
Rui-Jun ZHAO
;
Po LI
;
Bin DU
;
Fa-Jia YUAN
;
Shou-Min NIE
;
Jing-Xia CHENG
;
Yan-Hong LI
Author Information
1. 山西医科大学基础医学部
- Keywords:
Antimicrobial peptides;
Musca domestica;
SUP-B15;
Separation;
Purification;
Proliferation inhibition;
Apoptosis
- From:
Acta Parasitologica et Medica Entomologica Sinica
2015;(1):18-23
- CountryChina
- Language:Chinese
-
Abstract:
Crude extracts of antimicrobial peptides from 3rd larva of Musca domestica was purified with reversed phase high performance liquid hromatography ( RP-HPLC ) .Total 8 active protein was harvested and the inhibition effect of sup-b15 cell proliferation was conducted by survival and apoptosis assays.Sup-b15 cell proliferation was inhibited significantly evidenced by the survival rate ranged from 0.561 to 0.785 and apoptosis rate ranged from 9.79 to 35.94.Crude extracts induced more great inhibition on sup-b15 cell proliferation than purified proteins, which suggested the unclear mechanism was involved.
