IDENTIFICATION OF MOSQUITO-BORNE VIRUS ISOLATES IN YUNNAN PROVINCE, CHINA
- VernacularTitle:云南省蚊媒病毒分离物的初步鉴定
- Author:
Qiu-Min ZHAO
1
;
Xiao-Fang GUO
;
Shu-Qing ZUO
;
Hong-Ning ZHOU
;
Jiu-Song ZHANG
Author Information
1. 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071
- Keywords:
Getah virus;
Cytopathic effect;
RT-PCR;
Phylogenetic analysis
- From:
Acta Parasitologica et Medica Entomologica Sinica
2015;(1):12-17
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to identify viral isolates obtained from mosquitoes in Yunnan Province of China.The newly obtained isolates were identified by RT-PCR using alphavirus universal primers.Four of five isolates were from Culex tritaeniorhynchus and the rest was from Anopheles sinensis.They induced obvious cytopathic effect on C6/36, Vero and BHK-21 cell lines, respectively.Nucleotide sequence analyses showed that five isolates had high homology with Getah virus (GETV) YN0540, HB0234, M1 strains of China, and South Korea, LEIV 17741 MPR strains, and Sagiyama virus, with 96.4%-99.2% identities.The nucleotide sequence identifies among the five new isolates were 98.4%-100%.Phylogenetic analysis showed that the new isolates were clustered with the GETVs.These results confirmed that the new isolates belonged to GETV.
