APPLICATION OF AN OPTIMIZED METHOD FOR PCR DETECTION OF RABBIT COCCIDIA
10.3969/j.issn.1005-0507.2014.04.010
- VernacularTitle:兔球虫 PCR 检测方法的改进和应用
- Author:
Yun-Zhou WANG
1
;
Ge-Ru TAO
;
Yu-Juan CUI
;
Chao LI
;
Dan WANG
;
Ya-Qing JIAO
;
Xun SUO
;
Xian-Yong LIU
Author Information
1. 中国农业大学动物医学院,北京100193
- Keywords:
Rabbit coccidia;
WGA;
PCR;
Detection
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(4):268-272
- CountryChina
- Language:Chinese
-
Abstract:
Eimeria spp.of rabbits which cause great economic losses in rabbit industry belong to Phylum Apicomplexa. Since rabbit coccidia contain 11species, which have different pathogenicity between each other, it is necessary to identify the exact species in the feild samples to control rabbit coccidiosis.In order to improve the sensitivity and expand the application of PCR detection, we introduced the Whole Genome Amplification technique to preamplify the DNA template extracted from oocysts, then WGA product were used to amplify the ITS-1 sequence to identify species.The results showed that the optimized method guaranteed higher sensitivity which can identify the species based on single oocyst.Detection of 10 filed samples showed excellent specificity and consistency with morphological identification.By this method, the species that hard to discriminate under microscope can be accurately identified.Application of this method which provides higher sensitivity and specificity could make great effort to the rabbit coccidiosis investigation in practice.
