PRELIMINARY STUDY ON THE MECHANISMS OF RAPID EGRESS OF EIMERIA TENELLA SPOROZOITES FROM HOST CELLS
10.3969/j.issn.1005-0507.2014.04.009
- VernacularTitle:艾美耳球虫子孢子从宿主细胞中逸出机制的初步研究
- Author:
Xin-Lei YAN
1
;
Yong-Sheng JI
;
Xiu-Ling TIAN
;
Jing-Xia SUO
;
Xian-Yong LIU
;
Xun SUO
Author Information
1. 中国农业大学动物医学院,北京100193
- Keywords:
Eimeria tenella;
Sporozoites;
Ethanol;
Egress
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(4):263-267
- CountryChina
- Language:Chinese
-
Abstract:
Apicomplexan parasites infect nearly all vertebrate hosts even including humans and cause some severe diseases such as malaria, toxoplasmosis, cryptosporidiosis which are responsible for substantial economic losses. Like most intracellular pathogens, egress from host cells is a vital step of the apicomplexan parasites, which attracted attentions of many research groups.Unfortunately, as an important genus of Phylum Apicomplexa, little information of egress is known on Eimeria species which leads large amount of losses to poultry industry annually.In this report we used ethanol to induce egress of E.tenella M2e transgenic strain (EtM2e) sporozoites which express yellow fluorescent protein (YFP).Results showed that ethanol could induce egress of EtM2e sporozoites from infected Madin Derby Bovine Kidney ( MDBK) cells, and this process was depended on the mobility of the parasites.We also found that ethanol could also stimulate microneme protein discharge.Furthermore, both the course of egress and the secretion of microneme were controlled by the flux of intracellular Ca2+of the parasite.Taken together, our results preliminarily explained the mechanism of egress of eimerian parasites, which provided clues to the further study.
