STUDY OF THEAD APTIVE IMMUNE RESPONSE STIMULATED BY EIMERIA MITIS INFECTION IN CHICKEN
10.3969/j.issn.1005-0507.2014.04.007
- VernacularTitle:和缓艾美耳球虫激发宿主免疫应答的初步研究
- Author:
Xin-Ming TANG
1
;
Mei QIN
;
Jing-Xia SUO
;
Xiu-Ling TIAN
;
Xian-Yong LIU
;
Xun SUO
Author Information
1. 中国农业大学动物医学院,北京100193
- Keywords:
Eimeria mitis;
Immunogenicity;
Humoral immune response;
Cellular immune response
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(4):253-257
- CountryChina
- Language:Chinese
-
Abstract:
Eimeria mitis with low pathogenicity is easily ignored compared with other Eimeria spp.in chicken and the mechanism of difference immunogenicity between different strains is unclear.In this study, E.mitis ( Zhuozhou Strain) was used to analysis its immunogenicity by the oocysts output post each immunization.Moreover, We analyzed the humoral immune response and cellular immune response elicited by E.mitis infection via the serum IgY antibody titer detected by ELISA and IFN-γsecretion cell population post stimulated with E.mitis in peripheral blood mononuclear cell ( PBMC) by ELISPOT, respectively.Our results show that the oocyst output was relatively low and the IgY titer was still very low after the second immunization.However, high concentration of IFN-γwas detected in PBMC after stimulated with E.mitis post the second immunization 2 weeks.Our results indicated that strong cellular immune responses elicited by E.mitis could be contributed to its good immunogenicity and thus protect chicken from homologous infection.
