ANALYSIS ON THE LOCATION OF MICRONEME 2 PROTEIN IN SPOROZOITES OF EIMERIA SPP.BY INDIRECT IMMUNOFLUORESCENCE ASSAY
10.3969/j.issn.1005-0507.2014.04.004
- VernacularTitle:基于IFA技术检测微线体蛋白2在艾美耳属球虫的空间分布
- Author:
Xiu-Ling TIAN
1
;
Xin-Ming TANG
;
Mei QIN
;
Jing-Xia SUO
;
Xian-Yong LIU
;
Xun SUO
Author Information
1. 中国农业大学动物医学院,北京100193
- Keywords:
Eimeria spp.;
Monoclonal antibody;
Mic 2;
Location;
IFA
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(4):240-243
- CountryChina
- Language:Chinese
-
Abstract:
Microneme protein 2 ( Mic 2) is the structural protein of microneme of Eimeria spp.The secretion of Mic 2 is very important during the process of invading the host cells.In this study, we used a monoclonal antibody specific to E. tenella Mic 2 ( EtMic2) to detect the Mic 2 location in several Eimeria spp., i.e., E.tenella, E.mitis, E.stiedai and E. irresidua, by indirect immunofluorescence assay (IFA).The Mic 2 was located in the anterior region of the sporozoites among all Eimeria spp., which indicated that Mic 2 is highly conserved among Eimeria spp.and could be used as a marker protein when studying the location of Eimeria spp.proteins.
