DNA BARCODING IN SURVEYS OF RATS FROM HEIXIAZI ISLAND, SINO-RUSSIAN BORDER
- VernacularTitle:DNA 条形码技术在黑瞎子岛鼠类鉴定中的应用
- Author:
Run-Ji LIU
1
;
Rong-Bo ZHANG
;
Ming LI
;
Li-Juan LIU
;
Man-Xia HU
;
Jing WANG
;
Xiao-Mei CAO
;
Tian-Yu GUO
;
Xiao-Long ZHANG
Author Information
1. 安徽理工大学
- Keywords:
COⅠ gene;
DNA Barcoding;
Heixiazi Island;
Rat
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
To explore the application of DNA barcoding in the identification of rats, the mitochondrial cytochrome c oxidase subunit I genes of 41 rats from Heixiazi Island, belonging to 2 families, 3 generas and 4 species, were amplified.Blast comparisons were made in NCBI.The Intraspecific genetic distance and interspecific genetic distance were calculated with MEGA 5.0 based on Kimura double parameter model.The NJ tree was ultimately carried out.The results indicated that the interspecific genetic distance was remarkably greater than the intraspecific genetic distance .The phylogenetic analysis suggested that the lengths of interspecific branches were much longer than those of intraspecific branches.The rat samples of the same species constituted monophyletic groups .5 Clethrionomys rutilus rats were based on morphologic characters as Clethrionomys rufocanus.24 Microtus maximowiczii rats were based on morphologic characters as Microtus fortis.One Apodemus peninsulae rat was misidentified as Microtus fortis.Our research indicated that DNA barcode can not only identify correctly without morphological diagnoses, but also identify allied species.DNA barcoding based on COⅠgene can provide convenient and accurate identification of rats.
