CLONE AND SEQUENCING OF SODIUM CHANNEL GENE FROM FIELD CULEX PIPIENS PALLENS
10.3969/j.issn.1005-0507.2014.01.003
- VernacularTitle:自然种群淡色库蚊钠通道基因全长的克隆及序列分析
- Author:
Ming-Hui ZHAO
1
;
Xin RAN
;
Yan-De DONG
;
Xiao-Xia GUO
;
Ying-Mei ZHANG
;
Dan XING
;
Zhi-Ming WU
;
Chun-Xiao LI
;
Tong-Yan ZHAO
Author Information
1. 安徽医科大学
- Keywords:
Culex pipiens pallens;
Sodium channel;
Mutation;
Alternative splicing
- From:
Acta Parasitologica et Medica Entomologica Sinica
2014;(1):17-22
- CountryChina
- Language:Chinese
-
Abstract:
The sodium channel gene of Culex pipiens pallens collected from Hebei Province was cloned and sequenced using reverse transcription polymerase chain reaction ( RT-PCR ) .The alignment results are consistent with the sodium channel gene announced in GenBank.The study lays a foundation for development of the researches on mosquito resistance. Meanwhile, seven amino acid substitutions ( A32T, T336A, S342P, K535E, L1035F, N1595S and F1982L) and seven alternative splicing have been found in the sodium channel gene from this field Cx.pipiens pallens, including the classic resistant mutation L1014F ( L1035F).The relationship between these substitutions and resistance is still unknown and deserve further study.
