DETECTIONOFPSMT01INDIFFERENTSTAGEOF PARAGONIMUS SKRJABINI BY REAL-TIME FLUORESCENCE QUANTITATIVE QRT-PCR

VernacularTitle:qRT-PCR检测斯氏并殖吸虫不同虫期PsMt01基因表达研究
Author: Bei SONG ¹ ; Xi-Xiong KANG ; Jing-Xuan NIU ; Ying WANG ; Xi-Lin ZHANG
Author Information

1. 首都医科大学附属北京天坛医院实验诊断中心
Keywords: Paragonimus skrjabini; qRT-PCR; PsMt 01
From: Acta Parasitologica et Medica Entomologica Sinica 2014;(1):1-5
CountryChina
Language:Chinese
Abstract: PsMt 01 mRNA biological function was forecasted by using the method of real-time fluorescence quantitative (qRT-PCR) in different stage of Paragonimus skrjabini.Total RNA was extracted with Trizol reagents from the eggs, larvae, metacercaria, immature worm schistosomulum, adult stages respectively and transcribed reversely into cDNA.qRT-PCR was used to detect the expression level of PsMt 01 mRNA.The results of qRT-PCR displayed that the expression of PsMt01 mRNA increased gradually with the development of present, especially in the period of larva-5w (42.56 ±0.35) and larva-7w (44.12 ±0.56) within weeks, PsMt01 mRNA reached the peak level.Accordingly, we concluded that PsMt 01 may play an important role during the early P.skrjabini development stage, which may be associated with immune escape and organizing migration activities.