GENOMIC STRUCTURE AND EFFICACY PRESUMPTION OF THE PARTIAL PROPHENOLOXIDASE GENE(PPO1 AND PPO2) ON THE MALARIA VECTOR ANOPHELES SINENSIS
10.3969/j.issn.1005-0507.2012.03.003
- VernacularTitle:中华按蚊前酚氧化酶基因部分序列的基因结构和功能预测
- Author:
Wei-Long TAN
1
;
Zhong-Can WANG
;
Chang-Jun WANG
;
Min CAO
;
Jian ZHENG
;
Zhao-Jiu HAN
;
De-Sheng JIA
Author Information
1. 南京军区疾病预防控制中心
- Keywords:
Anopheles sinensis;
Prophenoloxidase;
Cloning
- From:
Acta Parasitologica et Medica Entomologica Sinica
2012;19(3):141-148
- CountryChina
- Language:Chinese
-
Abstract:
Prophenoloxidase, a melanin-synthesizing enzyme, is considered to be an important arthropod immune protein. In order to clone the prophenoloxidase gene of Anopheles sinensis, degenerative primers were designed according to the conserved protein fragments of prophenoloxidase gene from sibling species deposited on GenBank. RNA sequence of adult An. sinensis was reverse-transcripted into cDNA strand which was amplified by PCR to get the 600 bp target fragment, the partial prophenoloxidase cDNA sequence. The target fragment was cloned into PMD18-T vector and sequenced. The partial cDNA sequences of prophenoloxidase of An. sinensis was identified and named ASPPO1, ASPPO2 respectively. The homology blasted with An. gambiae ranged from 57.58% to 100%. The two sequences were submitted to NCBI with the accessing number: JX295575, JX295576. The rooted phylogenetic tree showed the homology with PPO2 of An. gambiae. The putative amino acid fragments were submitted to Swiss-model blasting with An. gambiae to simulate the 3D spatial structure. The QMEAN score of the model showed a further study was needed to ensure whether the two PPO genes were from the midgut and hemolymph against plasmodium or not.
