CLONE AND ANALYSIS ON GENE FRAGMENTS OF NEW MEMBERS OF CYP6 FAMILY FROM GENOME OF AEDES ALBOPICTUS
10.3969/j.issn.1005-0507.2001.01.005
- VernacularTitle:白纹伊蚊基因组中CYP6家族新成员基因片段的克隆与分析
- Author:
Yu WU
1
;
Jionglie HUANG
;
Guoli ZHOU
;
Xiaoxin LIU
;
Zuoliang ZHONG
Author Information
1. Sun Yet-sen University of Medical Sciences
- From:
Acta Parasitologica et Medica Entomologica Sinica
2001;8(1):23-30
- CountryChina
- Language:Chinese
-
Abstract:
Aim:To obtain new members'gene fragments of CYP6 family from Aedes albopictus. Method: A pair of degenerate primers according to the homologous amino acid sequences of CYP6A1,CYP6D1 in Musca domestica and CYP6E1 in Culex quinquefasciatus was designed and used to amplify CYP6 gene from genomic DNA of sensitive and deltamethrin-resistant strains of Ae.albopictus by PCR technique. Five DNA fragments sized as designed were obtained. These fragments were recombined by T-A ligation, and cloned into JM109.Five positive clones were selected to sequence. Homologous analysis on these deduced amino acid sequences were executed. Results: Five nucleotide sequences were obtained, of which the length is from 236bp to 240bp.The identities of these cloned sequences to other known members of CYP6 are from 23.1% to 53.8%,to CYP3 are from 25.6% to 43.9%,and to CYP4 are from 13.9% to 24.4%. Conclusion:These cloned sequences are gene fragments from new members of CYP6 family.
