DETECTION OF PLASMODIUM VIVAX BY NESTED PCR AMPLIFICATION OF DRIED BLOOD SPOTS ON FILTER PAPERS
10.3969/j.issn.1005-0507.2000.01.002
- VernacularTitle:套式PCR检测滤纸干血滴中间日疟原虫
- Author:
Chun NIU
1
;
Xinping ZHU
;
Lei ZHOU
;
Qiang LIU
Author Information
1. Capital University of Medical Sciences
- Keywords:
Plasmodium vivax;
nested-PCR;
blood filter paper;
Chelex
- From:
Acta Parasitologica et Medica Entomologica Sinica
2000;Ⅶ(1):7-10
- CountryChina
- Language:Chinese
-
Abstract:
Malaria parasites DNA was eluted from dried blood spot on filter paper by Chelex treatment and amplified by PCR to detect low parasitemia of Plasmodium vivax. Sensitivity and specificity of nested PCR assay were confirmed by amplifying a 121 bp DNA fragment of SSUrRNA gene of Plvivax.Density of about 25 parasites per ul of blood can be detected successfully.None of other three human malaria species(Plasmodium falciparum,Plasmodium malariae and Plasmodium ovale)samples was positive.The ease of collection and transport of filter paper specimens combined with the sensitive and specific detection of P.vivax by nested PCR suggest that this method might be a valuable tool for moleculr epidemiological study of P.vivax.
