IL-17A collaborating with TGF-β1 in regulating benign tracheal stenosis after tracheal injury in experimental dogs
10.3969/j.issn.1008-794X.2025.05.008
- VernacularTitle:白细胞介素-17A协同转化生长因子-β1调节犬气管损伤后良性气管狭窄
- Author:
Chun ZHAO
1
;
Yunfeng DENG
;
Wei SU
;
Hongyan DAI
;
Lusheng LIANG
;
Xueguang CAI
;
Song XU
;
Jun WANG
;
Xin YANG
;
Junren ZENG
Author Information
1. 650021 云南昆明 云南大学附属医院放射科
- Keywords:
tracheal stenosis;
interleukin-17A;
transforming growth factor-β1
- From:
Journal of Interventional Radiology
2025;34(5):487-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the effect of interleukin-17A(IL-17A)and transforming growth factor-β1(TGF-β1)on the benign tracheal stenosis after tracheal injury in experimental dogs.Methods The trachea stenosis model of healthy Beagle dogs was established by burning the middle part of trachea with electric snare under bronchoscopy guidance.A total of 21 dogs were divided into normal group(n=3,receiving normal feeding),molding group(n=12,after airway modeling every 3 dogs were sacrificed each week for 4 weeks),IL-17A suppression group(n=3,receiving Secukinumab after airway modeling),and IL-17A inhibitor+TGF-β1 inhibitor group(n=3,receiving Secukinumab and SB43154 after airway modeling).Bronchoscopy and CT scan were performed once a week,and the stenosis degree was calculated.RT-qPCR,immunohistochemistry,and HE staining of the obtained tracheal tissues were performed.Results Within 1-4 weeks after molding,in module-making dogs the degree of stenosis of the injured trachea gradually increased,and the expressions of ECM-related proteins,TGF-β1 and IL-17A were up-regulated.After treatment with IL-17A inhibitors,the inflammatory infiltration and granulation tissue hyperplasia were reduced and the early tracheal stenosis was improved(P＜0.05).The combination use of IL-17A inhibitor and TGF-β1 inhibitor had a better remission effect(P＜0.05).Conclusion IL-17A and TGF-β1 may synergistically affect the formation of tracheal stenosis.
