High expression of T-cell immunoglobulin and mucin domain-containing protein 3 predicting prognosis of non-small cell lung cancer by promoting monocyte migration
10.16098/j.issn.0529-1356.2025.06.009
- VernacularTitle:T细胞免疫球蛋白黏蛋白3高表达促进单核细胞迁移预测非小细胞肺癌预后
- Author:
Lu ZHOU
1
;
Si-Tong CHEN
;
Chun-Yan LI
;
Yan-Yan HUANG
;
Li-Yun XU
Author Information
1. 舟山医院检验中心
- Keywords:
T cell immunoglobulin and mucin domain-containing protein 3;
Monocyte;
Migration ability;
Non-small cell lung cancer;
Prognosis;
Flow cytometry;
Human
- From:
Acta Anatomica Sinica
2025;56(6):697-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of T cell immunoglobulin and mucin domain-containing protein 3(TIM-3)in peripheral blood mononuclear cells and its relationship with non-small cell lung cancer(NSCLC).Methods TIM-3 expression in monocytes from the peripheral blood of 116 NSCLC patients and 37 healthy volunteers was analyzed via flow cytometry.The expression of TIM-3 in macrophages from tissues was detected via multiplex immunohistochemical staining.Transwell migration experiments were used to detect the migration ability of monocytes.Real-time PCR and ELISA were used to detect the mRNA and protein expression of TIM-3 in monocytes.Results Compared with that in healthy volunteers,TIM-3 expression was upregulated in the peripheral blood monocytes of NSCLC patients(P＜0.05).TIM-3 was expressed mainly in macrophages in adjacent tissues and positively correlated with its expression in monocytes in NSCLC patients(P＜0.05).Furthermore,high TIM-3 expression promoted monocyte migration in NSCLC patients(P＜0.05),and high TIM-3 expression in peripheral blood monocytes indicated poor prognosis(P＜0.05).Conclusion TIM-3 expression in peripheral blood monocytes may be a useful indicator of the disease prognosis of NSCLC patients and TIM-3 could be a new target for immunotherapeutic strategies.
