Differentiation of bone marrow mesenchymal stem cells into cardiomyocytes induced by rehmannia glutinosa polysaccharide combined with basic fibroblast growth factor
10.16098/j.issn.0529-1356.2025.06.007
- VernacularTitle:地黄多糖联合碱性成纤维细胞生长因子诱导骨髓间充质干细胞向心肌细胞分化
- Author:
Wei-Hong LIANG
1
;
Hui-Ying YANG
;
Xin-Ru XU
;
Hao-Dan SUN
;
Yang LÜ
;
Hai-Ping WANG
Author Information
1. 河北北方学院基础医学院组织学与胚胎学教研室,河北 张家口 075000;河北北方学院干细胞与生殖生物学实验室,河北 张家口 075000
- Keywords:
Rehmannia glutinosa polysaccharide;
Basic fibroblast growth factor;
Phosphatiolylinositol 3-kinase/protein kinase B;
Bone marrow mesenchymal stem cell;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2025;56(6):681-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of pretreatment with rehmannia glutinosa polysaccharide(RGP)and basic fibroblast growth factor(FGF-2)alone or in combination on the differentiation of bone marrow mesenchymal stem cells(BMSCs)into cardiomyoid cells and the related mechanisms.Methods BMSCs pretreated with FGF-2 and RGP alone or in combination were cultured for 1,2,and 4 weeks.The expression levels of NKx2.5 and GATA-4 were detected by Real-time PCR.BMSCs pretreated with FGF-2 or RGP alone and PI3K/Akt signaling pathway inhibitor LY294002 were used to detect the expression levels of myocardial specific proteins and related pathway proteins by Western blotting.Results Compared with the blank control group,pretreatment with FGF-2 or RGP alone increased the expression rate of myocardial specific markers,and the effect of combined pretreatment with FGF-2 and RGP was more obvious(P＜0.05).Compared with pretreatment with FGF-2 or RGP alone,pretreatment with LY294002 combined with FGF-2 or RGP significantly down-regulated the expression of cardiac-specific proteins in BMSCs and inhibited the phosphorylation of Akt(P＜0.05).Conclusion Both FGF-2 and RGP can induce BMSCs to differentiate into cardiomyocyte-like cells by regulating PI3K/Akt signaling pathway,and the combination of FGF-2 and RGP has a better inductive effect.
