Protective effect of LncRNA SNHG12 on neuronal damage induced by oxygen glucose deprivation/reoxygenation by targeting miR-140-3p
10.16098/j.issn.0529-1356.2025.06.004
- VernacularTitle:LncRNA SNHG12靶向微小RNA-140-3p对氧葡萄糖剥夺/复氧诱导的神经元损伤的保护作用
- Author:
Chao TANG
1
;
Yan-Qun CAO
;
Chao-Liang CHEN
;
Ju-Xiang ZHOU
Author Information
1. 邵阳学院普爱医学院,湖南邵阳 422000
- Keywords:
Long non-coding RNA;
Small nucleolar RNA host gene 12;
MicroRNA-140-3p;
Oxygen glucose deprivation/reoxygenation;
Human cortical neuron
- From:
Acta Anatomica Sinica
2025;56(6):658-663
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of long non-coding RNA(lncRNA)small nucleolar RNA host gene 12(SNHG12)on neuronal damage induced by oxygen glucose deprivation/reoxygenation(OGD/R)by targeting microRNA(miR)-140-3p.Methods Human cortical neurons(HCN)were used to establish an OGD/R model,which was separated into OGD/R group,negative control(NC)group,SNHG12 group,SNHG12+miR-NC group,and SNHG12+miR-140-3p mimics group.Normal cultured HCN were also taken as the control(Ctrl)group.There were 6 repetitions in each group.Cell proliferation,apoptosis,lactate dehydrogenase(LDH)activity,mitochondrial membrane potential,lncRNA SNHG12 and miR-140-3p expression levels,and the interaction of lncRNA SNHG12 and miR-140-3p were detected.Results Compared with the control group,the survival rate of HCN,mitochondrial membrane potential,and lncRNA SNHG12 expression were lower in the OGD/R group,while the LDH activity,apoptosis rate,and miR-140-3p expression were higher(P＜0.05).Compared with the OGD/R group and NC group,the survival rate of HCN,mitochondrial membrane potential,and lncRNA SNHG12 expression were higher in the SNHG12 group,while the LDH activity,apoptosis rate,and miR-140-3p expression were lower(P＜0.05).Compared with the SNHG12+miR-NC group,the survival rate of HCN,mitochondrial membrane potential,and lncRNA SNHG12 expression were lower in the SNHG12+miR-140-3p mimics group,while the LDH activity,apoptosis rate,and miR-140-3p expression were higher(P＜0.05).Dual luciferase activity showed a targeted relationship between lncRNA SNHG12 and miR-140-3p(P＜0.05).Conclusion LncRNA SNHG12 may exert a protective effect against OGD/R-induced neuronal damage by inhibiting miR-140-3p.
