Combination of electroencephalogram/electromyography and fiber photometry recording of multiple brain regions in mice and its application in epilepsy models
10.16098/j.issn.0529-1356.2025.05.004
- VernacularTitle:小鼠脑电图/肌电图与多脑区光纤记录联用及其在癫痫模型中的应用
- Author:
Tong-Tong GAO
1
;
Chen CHEN
;
Wei-Yi YE
;
Mao DENG
;
Heng-Hao HOU
;
Qian CHEN
Author Information
1. 广州中医药大学中药学院,广州 510006;中科中山药物创新研究院,广东中山 528400
- Keywords:
Electrical signal;
Calcium signal;
Epilepsy;
Electroencephalogram;
Fiber photometry;
Mouse
- From:
Acta Anatomica Sinica
2025;56(5):533-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method of combining electroencephalogram(EEG)/electromyography(EMG)with multi-regional fiber photometry recording to simultaneously capture the changes of neuronal activity in the whole brain and specific brain regions during epileptic seizures.Methods The mouse head was divided into left and right regions based on the middle suture of the skull.EEG electrodes(EEG/EMG)were implanted in one side,while optical fibers were implanted in the striatum,hippocampus,entorhinal cortex,and thalamus on the contralateral side to simultaneously monitor EEG,EMG,and calcium signal dynamics.Results By combining EEG/EMG with multi-regional fiber photometry recording,differences in neuronal activity across brain regions,alongside EEG and EMG,were observed during different behavioral states.In a kainic acid(KA)-induced epilepsy model,abnormal synchronous neuronal discharges in the mouse brain were accompanied by calcium signal changes in the striatum,hippocampus,entorhinal cortex,and thalamus,with the earliest changes occurring in the hippocampus.Conclusion The combined use of EEG/EMG and multi-brain-region fiber photometry is successfully implemented in mice.This method synchronously recordes abnormal calcium signal changes across multiple brain regions,along with EEG and EMG,in the KA-induced epilepsy model.
