Differences of perineuronal nets and parvalbumin-positive neuron distribution across specific brain regions in young and aged mice
10.16098/j.issn.0529-1356.2025.05.002
- VernacularTitle:神经周围网和小清蛋白阳性神经元在不同年龄段小鼠特定脑区分布的差异
- Author:
Mei FU
1
;
Wei-Yi YE
;
Heng-Hao HOU
;
Qian CHEN
Author Information
1. 南方医科大学药学院,广州 510515;中科中山药物创新研究院,广东中山 528451
- Keywords:
Aging;
Perineuronal net;
Parvalbumin;
Inhibitory interneuron;
Neural plasticity;
Immunofluorescence;
Mouse
- From:
Acta Anatomica Sinica
2025;56(5):515-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the differences of perineuronal net(PNN)and parvalbumin(PV)-positive neuron distribution across specific brain regions between young and aged mice.Methods Brains from young(45 days)and aged(350 days)mice(n=4 per group)were fixed with 4％paraformaldehyde,sectioned(50 μm)using a vibratome,and stained with Wisteria floribunda agglutinin(WFA)and PV immunofluorescence.Quantitative analyses of PNN-positive and PV-positive neurons,along with PNN encapsulation of PV-positive neurons,were performed in the anterior cingulate cortex(ACC),somatosensory cortex barrel field layer 4(S1BF L4),striatum(STR),and hippocampal CA2 region.Results Aged mice exhibited no significant changes in PNN-positive or PV-positive neuron counts in ACC,S1BF L4,or STR compared to young mice,but showed significantly increased PNN encapsulation of PV-positive neurons.In hippocampal CA2,PNN-positive neurons increased significantly without PV-positive neuron alterations.Conclusion The differences in PNN-PV neuron interactions and PNN density exist in specific brain regions of young and aged mice.
