Construction and evaluation of intestinal mucosal epithelial-myofibroblast co-culture model
10.16098/j.issn.0529-1356.2025.04.008
- VernacularTitle:肠黏膜上皮-肌成纤维细胞共培养模型的构建与评价
- Author:
Peng-Xin YU
1
;
Ming-Qi WANG
;
Li-Na GUO
;
Yu YU
;
Xiu-Li WANG
Author Information
1. 大连医科大学基础医学院组织学胚胎学教研室,大连 116000
- Keywords:
Intestinal mucosal epithelium model;
Fibroin;
Villous-crypt;
Three-dimensional co-culture
- From:
Acta Anatomica Sinica
2025;56(4):438-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a three-dimensional bionic model of intestinal mucosal structure in vitro.Methods Using fibroin protein with villous-crypt structure as scaffold and Caco-2 and HT29-MTX-E12 as seed cells,the intestinal mucosal epithelial-myofibroblast co-culture model was constructed by three-dimensional co-culture with myofibroblasts in vitro.The growth,activity,histological characteristics and expression of functional genes of model cells were investigated through the detection of cell signature proteins and genes.Results 1.An intestinal mucosal epithelial-myofibroblast co-culture model was successfully constructed with good cell activity.2.Mucous secretion increased and muc2 gene expression was up-regulated in the co-culture model.Conclusion The co-culture of intestinal mucosal epithelial-myofibroblasts with villous-crypt structure can promote the functional differentiation of intestinal epithelial cells and better simulate the structure and function of intestinal mucosa in vivo.
