Therapeutic effect of remimazolam on cerebral ischemia-reperfusion injury rats by regulating the AMP-activated protein kinase/NOD-like receptor protein 3 signaling pathway
10.16098/j.issn.0529-1356.2025.04.007
- VernacularTitle:瑞马唑仑调节腺苷酸活化蛋白激酶/NOD样受体蛋白3信号通路对大鼠脑缺血再灌注损伤的治疗作用
- Author:
Teng FAN
1
;
Xiao-Fang LI
;
Dan SHEN
;
Hong-Wei ZHANG
;
Xiu-Qin YUE
Author Information
1. 新乡医学院第一附属医院麻醉与围术期医学科,河南新乡 453100
- Keywords:
Remimazolam;
Ischemia-reperfusion;
Brain injury;
AMP-activated protein kinase/NOD-like receptor protein 3 signaling pathway;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
2025;56(4):431-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of remimazolam(REM)on cerebral ischemia-reperfusion injury(CIRI)rats and the AMP-activated protein kinase(AMPK)/NOD-like receptor protein 3(NLRP3)signaling pathway.Methods One hundred rats were selected to construct the CIRI rat model(Mod)and stochastically separated into a Mod group,low,medium,and high dose remifentanil groups(REM-L,REM-M,REM-H),and high dose remifentanil+pathway inhibitor Compound C group(REM-H+Compound C),with 20 rats in each group.Another 20 healthy rats were included as the control(Ctrl)group.All rats were subjected to neurobehavioral scoring.The water content,infarct area,and oxidative stress indicators of brain tissue were detected.The morphology and apoptosis of brain tissue were observed by HE and TUNEL staining.Western blotting was applied to detect protein expression related to the AMPK/NLRP3 signaling pathway.Results Compared with the Mod group,with the increase of REM dose,the movement disorders in rats were alleviated,the overall structure of brain tissue gradually recovered,pathological damage was reduced,the area of cerebral infarction,brain water content,and apoptosis rate of brain tissue cells decreased,reactive oxygen species(ROS)level,malondialdehyde(MDA)content,and NLRP3 and Caspase-1 protein expression levels decreased,superoxide dismutase the(SOD)content and AMPK protein expression level increased(P＜0.05).Compared with the REM-H group,the REM-H+Compound C group showed aggravated motor disorders,and more severe pathological damage to brain tissue,the area of cerebral infarction,cerebral water content and apoptosis rate of brain tissue cells increased,the ROS level,MDA content and the protein expression of NLRP3 and Caspase-1 increased,while the content of SOD and the protein expression decreased(P＜0.05).Conclusion Remimazolam can enhance the antioxidant function of the body,reduce brain cell apoptosis,alleviate brain tissue injury,and thus have a certain protective effect on ischemia-reperfusion brain injury in rats,the mechanism of which may be related to the activation of the AMPK/NLRP3 signaling pathway.
