Effects of high-fructose diet on hippocampal neurometabolites and anxiety and depression-like behaviors in mice
10.16098/j.issn.0529-1356.2025.04.001
- VernacularTitle:高果糖饮食对小鼠海马脑区神经代谢物及焦虑与抑郁样行为的影响
- Author:
Shan-Shan ZHANG
1
;
Ying LIU
;
Yu-Wen ZHANG
;
He WANG
;
Wen-Sheng LI
;
Qiong LIU
Author Information
1. 复旦大学基础医学院人体解剖学与组织胚胎学系,上海 200032
- Keywords:
High-fructose diet;
Anxiety;
Hippocampus;
Neurometabolity;
Hydrogen proton magnetic resonance spectroscopy;
Mouse
- From:
Acta Anatomica Sinica
2025;56(4):381-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of short-term and long-term high-fructose diets on hippocampal neurometabolites and anxiety and depression-like behaviors in mice,revealing the potential mechanisms of high-fructose diets in mood disorders and providing a experimental basis for the prevention and treatment of related diseases.Methods C57BL/6J male mice were randomly divided into two groups,control group(standard diet,n=10)and experimental group(high-fructose diet,n=10).Four weeks(short-term)and eight weeks(long-term)later,each group of mice was examined for body weight and fasting blood glucose,and neurometabolites levels in the hippocampus were detected by hydrogen proton magnetic resonance spectroscopy(1H-MRS),followed by the open-field test,the forced-swimming test,and the tail-suspension test to evaluate anxiety-like and depression-like behaviors.Results High fructose diet for 4 weeks elevated glutamate levels and reduced glutathione and myo-inositol levels in mice,accompanied by shortened immobility time in the forced swim test.High fructose diet for 8 weeks not only led to abnormalities in body weight and glucose metabolism but also caused a reversal decrease in hippocampal glutamate levels and induced significant anxiety-like behaviors,and the decrease in hippocampal glutamate levels showed a significant negative correlation with the enhancement of anxiety-like behaviors.Conclusion Altered hippocampal glutamate levels may be a key contributing factor to the anxiety-like behaviors induced by long-term high-fructose diet.
