Method of differentiation of human induced pluripotent stem cells into high purity dopaminergic neurons in vitro
10.16098/j.issn.0529-1356.2025.03.014
- VernacularTitle:人诱导多能干细胞体外定向分化为高纯度多巴胺能神经元的方法
- Author:
Jie-Yi MENG
1
;
Xuan FANG
;
Man LI
;
Wei-Guang ZHANG
;
Chun-Hua CHEN
Author Information
1. 北京大学医学部基础医学院人体解剖学与组织学胚胎学系,北京 100083
- Keywords:
Dopaminergic neuron;
Substantia nigra pars compacta;
Induced pluripotent stem cell;
In vitro differentiation with high purity;
Real-time PCR;
Immunofluorescence;
Human
- From:
Acta Anatomica Sinica
2025;56(3):351-356
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore an experimental protocol for differentiating human-induced pluripotent stem cells(iPSCs)into highly pure midbrain dopaminergic(DA)neurons.Methods By optimizing a blend of small molecules and recombinant human growth factors,iPSCs were induced to differentiate into ventral midbrain floor plate DA progenitor cells and subsequently into mature substantia nigra pars compacta DA neurons.Throughout the differentiation process,Real-time PCR and immunofluorescent staining were utilized as a method for quality assessment.Results iPSCs firstly differentiate into dopaminergic precursor cells,and then gradually differentiate into DA neurons expressing tyrosine hydroxylase(TH).Conclusion The protocol successfully yields approximately high purity tyrosine hydroxylase-positive(TH+)DA neurons.This differentiation technique offers an effective cellular model for studying the physiological mechanisms and pathogenesis of Parkinson's disease,providing valuable insights for future research and potential therapeutic strategies.
