Mechanism of P-bodies recruiting NANOS3 during early oogenesis
10.16098/j.issn.0529-1356.2025.03.010
- VernacularTitle:早期卵子发生中P小体募集NANOS3的机制
- Author:
Jian-Wei GENG
1
;
Fei HE
;
Yi-Dan MA
;
Yong-Rui ZHOU
;
Xin-Yi MU
Author Information
1. 重庆医科大学基础医学院;重庆医科大学生殖与发育国际联合实验室
- Keywords:
NANOS3;
P-body;
Early oogenesis;
Immunofluorescence;
Neonated mouse
- From:
Acta Anatomica Sinica
2025;56(3):323-328
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between NANOS3 and P-bodies in oocytes and the mechanism of their interaction during early oogenesis.Methods The co-localization of NANOS3 and dead box helicase 6(DDX6)in day post postnatal 1(1dpp)mouse oocytes was observed by immunofluorescence,and the interaction between NANOS3 and DDX6 was detected by immunoprecipitation.NANOS3 and DDX6 full-length plasmids were constructed to transfect HEK293T cells,and the mechanism of their interaction was investigated by immunofluorescence and immunoprecipitation.NANOS3 transfected HeLa cells to investigate whether NANOS3 had the ability of liquid-liquid phase separation(LLPS)by live-cell imaging.The proteins recruited by P-bodies in early oogenesis were identified by DDX6-immunoprecipitation-mass spectrometry(DDX6-IP-MS).Results NANOS3 and DDX6 colocalized and interacted with each other in 1dpp mouse oocytes.However,the co-localization of NANOS3 and DDX6 was not observed in HEK293T cells that had been transfected,but co-immunoprecipitation still demonstrated an interaction between these two proteins.Besides,live-cell imaging revealed that NANOS3 exhibited dynamic fluid-like properties within cells,which may promote the formation of P-bodies through LLPS.Finally,DDX6-IP-MS revealed that DDX6 might recruit NANOS3 into P-bodies by binding to the NANOS3 interacting protein Pumilio.Conclusion NANOS3 serves as a specific component of P-bodies in neonatal oocytes and may be involved in the regulation of early oogenesis.
