Investigating mechanism of cinobufagin in gastric cancer treatment based on network pharmacology and bioinformatics
10.16098/j.issn.0529-1356.2025.01.006
- VernacularTitle:基于网络药理学与生物信息学探究华蟾酥毒基治疗胃癌的作用机制
- Author:
Hao ZHANG
1
;
Xue-Yan LI
;
Ling-Min LI
;
Bai-Yu JIAN
Author Information
1. 齐齐哈尔医学院多基因病研究所,黑龙江 齐齐哈尔 161006
- Keywords:
Cinobufagin;
Gastric cancer;
TCGA database;
Network pharmacology;
Molecular docking
- From:
Acta Anatomica Sinica
2025;56(1):43-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of cinobufagin(CBG)in treating gastric cancer based on network pharmacology combined with bioinformatics and molecular docking technology.Methods Active ingredients and potential targets of CBG in treating gastric cancer were collected from PubChem,TCMSP,and SwissTargetPrediction databases.Transcriptional data of gastric cancer samples were obtained from TGGA database,and gastric cancer-related targets were identified through differential gene analysis.Intersection of targets between CBG and gastric cancer diseases was subjected to Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Protein-protein interaction(PPI)network of common targets was constructed using STRING database,and core targets were selected using Cytoscape software.Molecular docking verification of core targets screened with SYBYL-X 2.1.1 software was conducted with CBG.Results CBG treatment of gastric cancer involved 59 targets,with 19 key targets identified.Key targets such as aurora kinase A(AURKA),cyclin-dependent kinase 1(CDK1),enhancer of zeste homolog 2(EZH2),hepatocyte growth factor receptor(MET),matrix metallopeptidase 3(MMP-3),progesterone receptor(PGR),prostaglandin-endoperoxide synthase 1(PTGS1),and thymidylate synthase(TYMS)which exhibited good binding activity with CBG and were closely associated with gastric cancer prognosis.Conclusion CBG may exert anti-gastric cancer effects through multiple targets and pathways.
