Effect of triptolide on the expression of Polo-like kinase-1 in CT26 colon cancer and its antitumor activity
10.16098/j.issn.0529-1356.2025.01.001
- VernacularTitle:雷公藤甲素下调CT26结肠癌细胞保罗样蛋白激酶-1发挥抑瘤活性
- Author:
Zhi-Hao LU
1
;
Xue-Ming LI
;
Yan-Ling JIANG
;
Xu ZHAO
;
Jing FENG
;
Jian LI
Author Information
1. 北京中医药大学中医学院组织学胚胎学教研室,北京 100029
- Keywords:
Triptolide;
CT26 cell line;
Tumor-bearing model;
Polo-like kinase-1;
Immunohistochemistry;
Mouse
- From:
Acta Anatomica Sinica
2025;56(1):4-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor effects of triptolide against CT26 colon cancer and its impact on the expression of Polo-like kinase-1(PLK-1)protein.Methods Forty clean grade BALB/c mice,each mouse was implanted with 1×106 CT26 cells into the dorsal side of the right forelimb to establish a tumor-bearing mouse model.Experimental animals were randomly divided into four groups,the tumor model group(saline control),the positive drug group[oxaliplatin,5 mg/(kg·d)],the low-dose triptolide group[50 μg/(kg·/d)],and the high-dose triptolide group[100 μg/(kg·d)].The drugs were administered through intraperitoneal injection(10 times in total,once every other day).The in vitro effects of the drugs on the proliferation,migration,invasion,and mitosis of CT26 cells were also assessed.Results Triptolide significantly inhibited the proliferation,migration,and invasion of CT26 colon cancer cells,and disrupted the separation of centrosomes and the correct arrangement of chromosomes during the prophase of mitosis in tumor cells.The binding energy of triptolide and PLK-1 protein was-7.1 kcal/mol,and it could down-regulate the expression of PLK-1 in CT26 cells.Conclusion Triptolide exerts its antitumor effects against CT26 colon cancer by downregulating the expression of PLK-1.
