Establishment and evaluation of a lipopolysaccharide-induced acute respiratory distress syndrome model in minipigs
10.11855/j.issn.0577-7402.0378.2025.0624
- VernacularTitle:脂多糖诱导急性呼吸窘迫综合征小型猪模型的构建与评估
- Author:
Chuang-Ye WANG
1
;
Ran WANG
;
Jian ZHANG
;
Ling-Xiao QIU
;
Bin QING
;
Heng YOU
;
Jin-Cheng LIU
;
Bin WANG
;
Nan-Bo WANG
;
Jia-Yu LI
;
Xing LIU
;
Shuang WANG
;
Jin HU
;
Jian WEN
;
Quan LI
;
Xiao-Ou HUANG
;
Kun ZHAO
;
Shuang-Lin LIU
;
Gang LIU
;
Mei-Ju WANG
;
Qing XIANG
;
Hong-Mei WU
;
Xiao-Rong SUN
;
Tao GU
;
Dong ZHANG
;
Qi LI
;
Zhi XU
Author Information
1. 陆军军医大学第二附属医院呼吸与危重症医学科/重大呼吸疾病精准诊疗与防控重庆市重点实验室/呼吸疾病研究重庆市高校重点实验室,重庆 400037
- Keywords:
acute respiratory distress syndrome;
pig;
animal models;
lipopolysaccharide
- From:
Medical Journal of Chinese People's Liberation Army
2025;50(9):1154-1161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a stable,reliable,and clinically relevant porcine model of endotoxin-induced acute respiratory distress syndrome(ARDS).Methods Ten 8-month-old male Bama minipigs were deeply sedated,followed by invasive mechanical ventilation and electrocardiographic monitoring.Lipopolysaccharide(LPS)was intravenously pumped at 600 μg/(kg·h)for 3 hours,then maintained at 15 μg/(kg·h)thereafter.Dynamic monitoring was performed at five time points after LPS injection(LPS 0,1,3,5,and 8 h),including arterial blood gas analysis and chest computed tomography(CT)scans.Pathological examination of lung tissues obtained via bronchoscopic biopsy(HE staining and transmission electron microscopy)was conducted.These indicators were comprehensively used to evaluate the success of the animal model.Results At 5 hours after LPS administration,8 minipigs developed symptoms such as skin cyanosis,elevated body temperature,and respiratory distress.The oxygenation index decreased to<300 mmHg.Chest CT scans showed diffuse pulmonary infiltrates.Histopathology revealed alveolar edema and hyaline membrane formation.Transmission electron microscopy demonstrated disruption of pulmonary blood-air barrier,depletion of lamellar bodies in type Ⅱ pneumocytes,inflammatory cell infiltration,and exudation of plasma proteins and fibrin.Compared with LPS 0 h,at LPS 8 h,the oxygenation index and arterial blood pH were significantly decreased(P<0.001),while blood lactic acid and serum potassium were significantly increased(P<0.05);serum calcium and base excess were significantly decreased(P<0.05),and the lung injury score based on HE-stained lung sections was significantly increased(P<0.01).Conclusion The porcine ARDS model established by continuous LPS injection can dynamically simulate the pathophysiological characteristics and typical pathological manifestations of clinical septic ARDS,making it an effective tool to study the pathogenesis,prevention,and treatment strategies of septic ARDS.
