Clinical value of plasma protein ARP2 in evaluating heatstroke prognosis based on proteomics
10.11855/j.issn.0577-7402.0433.2025.0805
- VernacularTitle:基于蛋白质组学探讨血浆蛋白ARP2预测热射病预后的临床价值
- Author:
Qing-Bo ZENG
1
;
Jing-Chun SONG
;
Nian-Qing ZHANG
;
Qing SONG
;
Long-Ping HE
;
Qing-Wei LIN
;
Lin-Cui ZHONG
;
Xing-Ping DENG
Author Information
1. 解放军联勤保障部队第 908 医院重症医学科,江西 南昌 330002;南昌市洪都中医院急重症二科,江西 南昌 330000
- Keywords:
heatstroke;
proteomics;
ARP2 protein;
prognosis
- From:
Medical Journal of Chinese People's Liberation Army
2025;50(9):1138-1145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate plasma protein expression changes in heatstroke patients using proteomics technology and to identify reliable prognostic biomarkers.Methods A retrospective analysis was conducted on 20 heatstroke patients hospitalized at the 908th Hospital of the Chinese PLA Joint Logistics Support Force from July 2022 to February 2024.Patients were divided into survival(n=16)and death groups(n=4)based on 28-day outcomes.Fasting venous blood samples were collected from both groups for proteomic analysis.Liquid chromatography-mass spectrometry(LC-MS/MS)was used to identify and screen differentially expressed proteins.Kyoto Encyclopedia of Genes and Genomes(KEGG)and Gene Ontology(GO)enrichment analyses were performed.LASSO regression was applied to screen key prognostic biomarkers,and receiver operating characteristic(ROC)curve analysis was used to evaluate their predictive value.Results A total of 27 upregulated and 90 downregulated proteins were identified between the two groups.These proteins were primarily involved in biological processes such as immune responses,complement activation,and metabolic processes.LASSO regression analysis indicated that actin-related protein 2(ARP2),cysteine-rich scavenger receptor type 1 M130(CD163),and catalase(CAT)could serve as effective biomarkers for evaluating heatstroke prognosis.ROC curve analysis demonstrated that ARP2 had higher diagnostic efficacy(AUC=0.98,sensitivity=0.80,specificity=1.00)compared to CD163(AUC=0.94,sensitivity=0.76,specificity=1.00),CAT(AUC=0.96,sensitivity=0.67,specificity=1.00),and acute physiology and chronic health evaluation Ⅱ(APACHE Ⅱ)score(AUC=0.79,sensitivity=0.44,specificity=1.00).Conclusion Elevated plasma ARP2 level has good clinical value for predicting poor prognosis in heatstroke patients.
