Effects of moderate static magnetic field exposure on emotional behavior and brain damage related molecules in mice
10.11855/j.issn.0577-7402.0165.2024.1125
- VernacularTitle:中强静磁场暴露对小鼠情绪行为及脑损伤相关分子的影响
- Author:
Xue-Jia WANG
1
;
Xue-Feng YANG
;
Yu-Meng YE
;
Yong-Yi WANG
;
Yan-Hui HAO
;
Hong-Yan ZUO
;
Feng-Song LIU
;
Yang LI
Author Information
1. 河北大学生命科学学院,河北 保定 071002;军事科学院军事医学研究院辐射医学研究所,北京 100850
- Keywords:
static magnetic field;
anxiety;
blood flow;
brain injury;
inflammation
- From:
Medical Journal of Chinese People's Liberation Army
2025;50(5):592-598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of a 100 mT static magnetic field(SMF)on emotional behavior and brain damage-related molecules in mice.Methods Fifty-eight C57BL/6N mice were randomly divided into control group(n=25)and observation group(n=33).Mice in observation group were exposed to a 100 mT SMF for 0.5 h/d over 14 consecutive days,while mice in control group underwent pseudo-exposure.On the 7 and 14 days of exposure,anxiety-like behavior was assessed using open field and elevated plus maze tests.Cerebral blood flow was monitored using laser speckle imaging,and the levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-4,central nervous system specific protein β(S100β),neuron-specific enolase(NSE),and brain-derived neurotrophic factor(BDNF)were measured by radioimmunoassay.BDNF expression in the brain was detected by immunofluorescence.Results On the 7 and 14 days of SMF exposure,the open field and elevated plus maze tests showed no statistically significant differences between observation and control groups in the frequencies,durations,and distance entering the central area of the open field and the open arm of the elevated plus maze(P>0.05).Laser speckle imaging revealed no significant difference in cerebral cortical perfusion compared with pre-exposure period(P>0.05).The results of radioimmunoassay showed that compared with control group,on the 7 d of SMF exposure,the serum IL-1β,NSE and S100β levels were significantly increased(P<0.05),the serum BDNF level was significantly decreased(P<0.05),and the IL-1β and TNF-α contents in brain tissues were significantly increased in observation group(P<0.01).On the 14 d of SMF exposure,serum IL-1β,TNF-α,NSE,and S100β levels were significantly increased(P<0.05,P<0.0001),and the brain IL-1β and TNF-α levels were significantly increased(P<0.01)in observation group.No statistically significant differences were found in anti-inflammatory cytokine IL-4 level of serum and brain tissue or BDNF content of brain tissue between the two groups(P>0.05).Conclusion Continuous exposure to a 100 mT SMF for 14 d at 0.5 h/d induces neuroinflammation and brain damage in mice,without inducing anxiety-like behavior.
