Effect and mechanism of the total paeony glycosides on spinal cord apoptosis in rats with spinal cord injury
10.11855/j.issn.0577-7402.2020.12.04
- VernacularTitle:赤芍总苷对脊髓损伤大鼠脊髓细胞凋亡的影响及机制
- Author:
Zong-Jin YUE
1
;
Ru-Yin LIU
;
Lu YU
;
Xin-Li WANG
;
Zhong-Kai FENG
;
Xi-Bin WANG
;
Yun-Peng LI
;
Da-Yong XU
Author Information
1. 河南省中医院脊柱科,郑州 450000
- Keywords:
total paeony glycoside;
spinal cord injury;
spinal cord cell;
oxidative stress;
cell apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2020;45(12):1238-1241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of total paeony glycosides(TPG)on spinal cord apoptosis in rats with spinal cord injury(SCI).Methods A total of 18 SD rats were randomly divided into control group,SCI group and TPG group(6 each).The SCI rat model was established using Allen method.For rats in control group,the spinal cord was sutured directly after laminectomy without spinal cord injury;those in TPG group were intraperitoneally injected with 25 mg/kg TPG every 24 hours after the establishment of SCI model,while those in SCI group and control group were intraperitoneally injected with the same volume of normal saline for 28 days.The rats'behavioral score were evaluated using Basso-Beattie-Bresnahan(BBB)method.The apoptosis of spinal cord cells was measured by flow cytometry.Western blotting was performed to detect the expression levels of cell apoptotic protein cleaved caspase-3 and cleaved caspase-9.The contents of MDA,SOD and LDH in the supernatant of spinal fluid were quantified with corresponding kits.Results Compared to those in control group,the BBB score decreased significantly,while the apoptosis rate of spinal cord cells and the expression levels of cleaved caspase-3 and cleaved caspase-9 were obviously elevated,and the contents of MDA and LDH increased,SOD decreased in the supernatant of spinal fluid in the rats of SCI group(P<0.05).Compared to those in SCI group,after treatment with TPG,the BBB score increased significantly,the apoptosis rate of spinal cord cells and the expression levels of cleaved caspase-3 and cleaved caspase-9 decreased obviously,while the contents of MDA and LDH decreased,and SOD increased in the supernatant of spinal fluid of the rats in SCI group(P<0.05).Conclusion TPG inhibits the apoptosis of spinal cord cells in SCI rats,which may related to the function of TPG in regulating oxidative stress.
