Association of MTHFR C677T gene polymorphism with serum Hcy level and subtypes of ischemic stroke
10.16352/j.issn.1001-6325.2025.12.1614
- VernacularTitle:MTHFR C677T基因多态性与血清Hcy水平及缺血性脑卒中亚型相关
- Author:
Yuchao ZHANG
1
;
Mingzhang XIE
;
Xiaochun ZHOU
;
Mengmeng ZHAO
;
Chenyan YANG
;
Yanxuan LIU
Author Information
1. 新乡医学院第一附属医院 遗传科,河南 新乡 453100
- Keywords:
homocysteine;
MTHFR C677T gene;
gene polymorphism;
ischemic stroke
- From:
Basic & Clinical Medicine
2025;45(12):1614-1618
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between methylenetetra-hydrofolatereductase(MTHFR)gene polymorphism and serum homocysteine(Hcy)level and subtypes of ischemic stroke(IS).Methods The study was conducted according to the matched principle of case-control design,310 patients with IS and 330 healthy people during the same period were selected as the case group and the control group.Recycling enzyme method and the real-time fluorescent quantitative PCR(RT-qPCR)method were used to detect the level of serum Hcy and the genotypes of MTHFR C677T,respectively.Results There was a significantly difference in MTHFR C677T genotype and allele frequency between the case and control groups(P＜0.05).The correlation analysis with different subtypes indicated that the frequencies of CT genotype(38.02%),TT genotype(10.74%),and T allele(29.75%)were significantly different in the LAA group(OR=1.662,95%CI:1.058-2.608,P＜0.05;OR=2.373,95%CI:1.110-5.073,P＜0.05;OR=1.663,95%CI:1.190-2.323,P＜0.05);The frequencies of TT genotype(10.53%)and T allele(27.30%)in SAO group were also significantly different(OR=2.130,95%CI:1.046-4.336,P＜0.05;OR=1.474,95%CI:1.075-2.021,P＜0.05).Further analysis of serum Hcy level showed that LAA group(19.55±5.61)μmol/L and SAO group(16.37±5.20)μmol/L were significantly higher than the control group(14.46±4.61)μmol/L(P＜0.001);Among the patients of both subtypes the serum Hcy levels in those with CT genotypes and TT genotypes were significantly higher than those in patients of CC genotypes(P＜0.001).Conclusions The gene polymorphism of MTHFR C677T has a significant effect on Hcy level in pa-tients with LAA and SAO stroke.
