Knockdown of PIAS3 alleviates glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in rat cardiomyocyte cell line H9c2
10.16352/j.issn.1001-6325.2025.12.1593
- VernacularTitle:敲低PIAS3缓解血糖波动诱导的大鼠心肌细胞系H9c2氧化应激及线粒体功能障碍
- Author:
Yongxia CHENG
1
;
Long YU
;
Huamin LI
;
Shuo ZHAO
;
Yiyang ZHANG
;
Guibo LIU
Author Information
1. 牡丹江医科大学 第一临床医学院 病理教研室,黑龙江 牡丹江 157011
- Keywords:
PIAS3;
glucose fluctuation;
oxidative stress;
mitochondrial dysfunction
- From:
Basic & Clinical Medicine
2025;45(12):1593-1599
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PIAS3 on glucose fluctuation-induced oxidative stress and mito-chondrial dysfunction in rat cardiomyocytes.Methods H9c2 were cultured in vitro,and divided into normal glucose control group(Control),mannitol-induced osmotic pressure control group(MG),constant high glucose group(HG),intermittent hyperglycemia group(IHG),IHG+siRNA NC group,and IHG+PIAS3 siRNA group.Cell proliferation was assessed using CCK-8 assay.LDH release,MDA and GSH levels,as well as SOD activity,were detected using corresponding kits.Mitochondrial membrane potential was evaluated via JC-1 staining combined with flow cytometry.ROS levels in cells and mitochondria were determined using DCFH-DA and MitoSOX staining,re-spectively.Protein expression of PI3K,p-PI3K,AKT,and p-AKT was analyzed by Western blot.Results Com-pared with the control group,intermittent hyperglycemia promoted oxidative stress and mitochondrial dysfunction,significantly upregulated PIAS3 expression(P＜0.001)and downregulated p-PI3K and p-AKT protein levels(P＜0.001).Knockdown of PIAS3 significantly alleviated oxidative stress and mitochondrial dysfunction induced by glucose fluctuations,and increased p-PI3K and p-AKT protein levels(P＜0.001).Conclusions Knockdown of PIAS3 may alleviate glucose fluctuation-induced oxidative stress and mitochondrial dysfunction in ratcardiomyocytes by activating the PI3K/AKT signaling pathway.
